Comparison of loop-mediated isothermal amplification (LAMP) and real-time polymerase chain reaction methods using reverse transcriptase (qRT-PCR) as molecular diagnostic methods of Sars Cov 2

Authors

  • George Adán Galdos Rodríguez Escuela de Posgrado, Universidad Católica de Santa María, Arequipa, Perú. Egresados de la Universidad Católica de Santa María, Arequipa, Perú
  • Andrea del Rosario Atahualpa Manrique Egresados de la Universidad Católica de Santa María, Arequipa, Perú
  • Dolly Solange Zeballos Carbajal Escuela de Posgrado, Universidad Católica de Santa María, Arequipa, Perú. Hospital III Goyeneche, Arequipa, Perú

DOI:

https://doi.org/10.35434/rcmhnaaa.2023.163.2142

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Author Biographies

George Adán Galdos Rodríguez, Escuela de Posgrado, Universidad Católica de Santa María, Arequipa, Perú. Egresados de la Universidad Católica de Santa María, Arequipa, Perú

1. Médico cirujano

2. Ingeniero biotecnólogo

Andrea del Rosario Atahualpa Manrique, Egresados de la Universidad Católica de Santa María, Arequipa, Perú

1. Médico cirujano

Dolly Solange Zeballos Carbajal, Escuela de Posgrado, Universidad Católica de Santa María, Arequipa, Perú. Hospital III Goyeneche, Arequipa, Perú

1. Médica especialista en Patología Clínica

References

Kitajima H, Tamura Y, Yoshida H, Kinoshita H, Katsuta H, Matsui C, et al. Clinical COVID-19 diagnostic methods: Comparison of reverse transcription loop-mediated isothermal amplification (RT-LAMP) and quantitative RT-PCR (qRT-PCR). J Clin Virol. 2021;139:104813.

Nguyen T, Duong Bang D, Wolff A. 2019 Novel Coronavirus Disease (COVID-19): Paving the Road for Rapid Detection and Point-of-Care Diagnostics. Micromachines. 2020;11(3):306.

Teymouri M, Mollazadeh S, Mortazavi H, Naderi Ghale-Noie Z, Keyvani V, Aghababaei F et.al. Recent advances and challenges of RT-PCR tests for the diagnosis of COVID-19. Pathol Res Pract. 2021;221:153443.

Xiao Yan. Comparison of three TaqMan real-time reverse transcription-PCR assays in detecting SARS-CoV-2. J Virol Methods. 2021;288:114030.

Avetyan, Diana. SARS-CoV-2 detection by extraction-free qRT-PCR for massive and rapid COVID-19 diagnosis during a pandemic in Armenia. J Virol Methods. 2021;295:114199.

Muller, Meredith. Practical strategies for SARS-CoV-2 RT-PCR testing in resource- constrained settings. Diagn Microbiol Infect Dis. 2021;101(2):115469.

Desmet T, Paepe P, Boelens J, Coorevits L, Padalko E, Vandendriessche S, Leroux-Roels I, Aerssens A, Callens S, Braeckel EV, Malfait T, Vermassen F, Verhasselt B. Combined oropharyngeal/nasal swab is equivalent to nasopharyngeal sampling for SARS-CoV-2 diagnostic PCR. BMC Microbiol. 2021;21(1):31.

Thompson D, Lei Y. Mini review: Recent progress in RT-LAMP enabled COVID-19 detection. Sens Actuators Rep. 1 de noviembre de 2020;2(1):100017.

Kashir J, Yaqinuddin A. Loop mediated isothermal amplification (LAMP) assays as a rapid diagnostic for COVID-19. Med Hypotheses. 2020;141:109786.

Wei S, Suryawanshi H, Djandji A, Kohl E, Morgan S, Hod EA, et al. Field- deployable, rapid diagnostic testing of saliva for SARS-CoV-2. Sci Rep. 2021;11(1):5448.

Published

2023-09-25

How to Cite

1.
Galdos Rodríguez GA, Atahualpa Manrique A del R, Zeballos Carbajal DS. Comparison of loop-mediated isothermal amplification (LAMP) and real-time polymerase chain reaction methods using reverse transcriptase (qRT-PCR) as molecular diagnostic methods of Sars Cov 2. Rev. Cuerpo Med. HNAAA [Internet]. 2023 Sep. 25 [cited 2024 May 16];16(3). Available from: http://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/2142